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Southern blots of catalase-defective mutants. Shown are the Southern blots of HindIII-digested genomic DNA from the wild type, Y4Nal (lane 2), and catalase-negative mutants, Aa1393 (lane 3), Aa1394 (lane 4), and Aa1395 (lane 5). Lane 1 contains digoxigenin-labeled DNA molecular weight markers (shown in kilobases) (Boehringer Mannheim). Probes were digoxigenin-labeled DNA containing the kan gene (A) or the katA gene (B).
