Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Properties of primers used in the construction of pVJT128 and pVJT131 plasmids
| Primer | Sequence and salient features | Annealing position |
|---|---|---|
| OKD110 |  |
3′ end of the Tn903 kanamycin gene |
| OKD115 | 5′ end of the Tn903 kanamycin gene beginning with the second codon | |
| OKD121 | Upstream of the kan′ gene and inverted repeat sequence | |
| OKD122 | Internal to the kan′ gene | |
