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Denaturation of phage DNA. DNA denatured with 0.2 M NaOH (+) and an untreated control (−) were resolved on a 0.5% agarose gel. The denatured phage DNA did not “snap back” and regenerate a double-stranded DNA molecule, as did the denatured covalently closed linear plasmid lp17. The arrow indicates single-stranded DNA products generated by the denaturation of non-covalently closed double-stranded DNA. The gel was stained with EtBr. Molecular sizes are in kilobase pairs.
