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. 2023 Jun 29;3(7):1952–1964. doi: 10.1021/jacsau.3c00198

Figure 2.

Figure 2

Backbone modifications of peptide nucleic acids (PNAs) with tetrahydrofuran (thf) and cyclopentane (cp). (A) Structural schematic of aegPNA (top left), thyclotide (top right), cpPNA (bottom left), and mixPNA (bottom right). Nine-nucleobase sequences representing aegPNA 1a, thyclotide 3i, cpPNA 2a, and mixPNA 4a. B* represents thf residue, B# represents cp residue, and AEEA = 2-(2-aminoethoxy)ethoxyacetyl group. (B) HPLC chromatogram of a mixture of aegPNA 1a, cpPNA 2a, thyclotide 3i, and mixPNA 4a (∼1:1:1:1 ratio). (C) CD spectra of unhybridized (single-strand) aegPNA 1a, thyclotide 3i, cpPNA 2a,37 and mixPNA 4a (50 μM in water).