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. 2023 Jun 29;3(7):1952–1964. doi: 10.1021/jacsau.3c00198

Figure 8.

Figure 8

11-thf and 14-thf thyclotides (11b and 11d) inhibit miR-21. (A) AntimiR-21 aegPNA 11a, antimiR-21 thyclotides 11b and 11d, and scramble thyclotides 11c and 11e for RT-qPCR of miR-21. B* represents thf residue, and X represents 2-aminoethoxy-2-ethoxy acetic acid (AEEA linker). (B) RT-qPCR of miR-21 expression in HCT116 cells treated with 1 or 5 μM scramble thyclotide 11e. miR-21 expression was normalized to non-treated cells. N = 3. (C) RT-qPCR of miR-21 expression in HCT116 cells treated with 1 μM antimiR-21 aegPNA 11a. miR-21 expression was normalized to scramble thyclotide 11e-treated cells. N = 3. (D) RT-qPCR of miR-21 expression in HCT116 cells after treatment at varying concentrations of antimiR-21 thyclotide 11b and 11d. N = 3. Quantification was normalized to scramble thyclotide 11c or 11e. All RT-qPCRs used the combination of miR-25-5p and miR-93-5p as endogenous controls. The difference between miR-21 expressions after thyclotides 11b and 11d treatments was statistically significant using a one-tail paired t-test at 1 μM (P = 0.0411) and 2 μM (P = 0.0033).