FIG. 1.

Schematic diagrams of rocF constructs used in this study. pBS-rocF::aphA3 and pILL236-2 contain the aphA3 kanamycin-resistant cassette. Thick arrows denote the direction of transcription. Restriction enzyme abbreviations: B, BamHI; C, ClaI; E, EcoRI; H, HindIII; P, PstI. Thick lines refer to vector sequences. The symbols  ‘  and  ’  refer to truncations at the 5′ or 3′ end, respectively. +1 refers to the first nucleotide in the coding region of rocF. Thin arrows denote primers used for PCR confirmation and sequencing of rocF mutants. See Table 1 for primer sequences, Table 2 for the PCR strategy, and Fig. 2B for the results.