TABLE 3.

Rates of amino acid catabolism determined for H. pylori cells of WT and rocF mutant strains (n = 4)^a

Strain	Mean rate of enzyme activity (nmol/min/mg of protein) ± SD
	Arginase	Asparaginase	Aspartase	Glutaminase	Serine dehydratase
26695	33 ± 4	19 ± 3	8 ± 2	66 ± 6	42 ± 7
26695 rocF::aphA3	UDb	19 ± 3	8 ± 2	62 ± 8	19 ± 3c
N6	51 ± 5	35 ± 6	11 ± 3	91 ± 10	37 ± 8
N6 rocF::aphA3	UD	20 ± 4	12 ± 3	80 ± 11	14 ± 3c
N6 236-2	UD	25 ± 4	11 ± 2	85 ± 10	25 ± 5d
SS1	54 ± 5	32 ± 3	17 ± 3	81 ± 8	50 ± 7
SS1 rocF::aphA3	UD	28 ± 5	11 ± 3	75 ± 8	17 ± 3e
SS1 236-2	UD	24 ± 3	15 ± 2	75 ± 6	32 ± 6e

^aBacteria were suspended in a phosphate (20 mM; pH 7)–NaCl (115 mM)–KCl (15 mM) buffer plus one of the following amino acids: l-arginine (100 mM), l-asparagine (80 mM), l-aspartate (80 mM), l-glutamine (80 mM), or l-serine (80 mM) and were measured for enzyme activity by NMR (see Materials and Methods). 

^bUD, undetectable. P < 0.0001 compared with its isogenic WT strain. 

^cP < 0.005 compared with its isogenic WT strain. 

^dP < 0.05 compared with its isogenic WT strain. 

^eP < 0.01 compared with its isogenic WT strain.