FIG. 2.

Secondary structure of the SP82 moiety of SP82-ermC RNA. The sequence shown is the one transcribed in vitro by T7 RNA polymerase. In vivo RNA contains an additional 2 nt (GU) at the 5′ end. Numbering is from the in vitro transcriptional start site. The 4-nt deletions in stems 2 and 3 are shown in boldface. The CAGC sequence that was deleted in stem 3 was replaced with an A to create an AflII site. The boxed UAG sequence in the loop portion of stem 2 is the stop codon for the putative SP82 gene 60 coding sequence.