Northern blot analysis of SP82-ermC RNA, using the A and B riboprobes. RNA was isolated from wild-type (+) and pnpA (−) strains containing the wild-type SP82 sequence (pYH199), the 4-nt deletion in stem 2 (pYH204), and the 4-nt deletion in stem 3 (pYH205). Values to the left are molecular sizes in nucleotides. The location of the 220-nt RNA detected in the strain carrying pYH205, which encodes the SP82-ermC RNA that is not cleaved by Bs-RNase III, is indicated by the open arrow to the right of the blot probed with the B riboprobe.