FIG. 7.

Analysis of 110-nt RNA. (A) Northern blot analysis of RNA from wild-type and pnpA strains carrying pYH199, which were separated on a sequencing gel. The probe was the B riboprobe (Fig. 1B). The leftmost lanes show a sequencing reaction used as a molecular size marker (sizes in nucleotides are indicated at the left). The figure is a composite of two exposures of the same filter. (B) Reverse transcriptase analysis of RNA isolated from wild-type and pnpA strains carrying pYH199, using primer 1 (left) or primer 2 (right). The four leftmost lanes in each panel show sequencing reactions used as molecular size markers (sizes in nucleotides are indicated at the left). Lanes: 1, wild-type strain with no plasmid; 2, wild-type strain with pYH199; 3, pnpA strain with pYH199.