GPL profiles of M smegmatis colony morphology variants. From left to right mc2155, Sm-1 (smooth), and six rough strains, Rg-1, Rg-2, Rg-3, Rg-4, Rg-5, and Rg-6. Approximately equal dry weights of lipid extract were loaded in each lane. The TLC was developed in chloroform-methanol-water (90:10:1), dried, sprayed with 10% H2SO4 in ethanol, and heated to 120°C.