Fig. 7.

37-mer peptide IC50 treatment apoptotic effect on SNU449 at 24 h. A Treated cells exhibited early-stage apoptotic death, no effect on late-stage apoptosis/necrosis, with a significantly lower live cell count (*** P < 0.001, n = 3). B Representative images of annexin V assay microscopy. Merged images showing overlapping green and red signals in untreated control and 37-mer peptide IC50 treated fields. Peptide treatment appeared to increase the rate of early apoptosis in SNU449 cells, compared to untreated cells. Additionally, PI staining was visually similar whether cells were treated or not. Cell images are processed at magnification scale of 50 μm