Fig. 6.

Increase is Aβ burden in brains of 4 and 6-month-old 5xFAD TAU vs. 5xFAD mice. Hippocampal sections from WT, 5xFAD and 5xFAD TAU mice were stained using 6E10 antibody. A Representative images of 4- and 6-month-old mice, Scale bar = 100 µm and B quantification of 4- and 6-month-old transgenic mice. Statistical analysis was performed as area of 6E10 staining vs hippocampal region, results are presented in mean ± SEM, n = 4–6; C, D Congophilic plaque histological analysis of brains of 4- and 6-month-old transgenic mice. Hippocampal sections from mice were stained with Congo red (statistical analysis was performed using one-way ANOVA, results are presented in mean ± SEM n = 6, Scale bar = 100 µm). E Staining with OC antibody, specifically recognizes fibrils amyloid in the cortex of TAU, 5xFAD and 5xFAD TAU mice Scale bar = 100 µm, and F ELISA assessment of soluble Aβ42 and insoluble Aβ42 vs total brain and the Aβ ratio between insoluble to soluble Aβ42 in 5xFAD TAU vs 5xFAD mice (n = 6–7)