Fig. 8.

Expression of mutated TAU in astrocytes impaired their morphology and activity. Astrocytes were isolated from mice expressing mutated TAU and compared to WT C57BL6/J mice. Scale bar 50 µm. A Co-staining with GFAP (red) and NeuN (green) of brain from 3-month-old WT and TAU mice; B RT-PCR analysis of GFAP, S100b and VEGF from primary astrocytes from WT and TAU mice (n = 2 repeats per group, each repeat is based on 2 mouse brains); C VEGF protein levels in Tau mutated primary astrocytes compared to WT astrocytes. Protein expression was determined and was taken from 1- to 3-day-old WT vs. TAU newborn mice (n = 3 repeats per group, each repeat is based on 2 mouse brains. Representative bands and Protein quantification. Statistical analysis was performed using Student’s t-test, results are presented in mean (% fold increase of control); D FACS analysis of uptake of fluorescent Aβ42 by adult astrocyte from WT and TAU mice (n = 8–9); E mRNA analysis of TGF-β1 expression by adult astrocyte from WT and TAU mice (n = 4–5); and F mRNA expression levels analysis from adult microglia isolated by CD11b magnetic beads from 3-month-old WT and TAU mice (n = 3–4, each repetition is based on 2 mice; *p < 0.05; **p < 0.01, ***p = 0.005)