TABLE 2.
Substrate specificity and kinetic parameters of purified CelF
| P-β-glucoside | Km (mM) | Vmax (μmol min−1 mg−1) |
|---|---|---|
| 4MUβG6P | 0.30 ± 0.02 | 3.35 ± 0.08 |
| Arbutin-6Pa | 0.35 ± 0.04 | 2.13 ± 0.08 |
| pNPβG6P | 0.44 ± 0.04 | 1.33 ± 0.05 |
| Salicin-6Pb | 0.44 ± 0.09 | 1.38 ± 0.09 |
| Cellobiose-6P | 1.30 ± 0.09 | 1.74 ± 0.05 |
| MethylβG6P | 2.22 ± 0.21 | 0.56 ± 0.03 |
| Gentiobiose-6Pc | 12.50 ± 1.26 | 1.11 ± 0.11 |
a
4-hydroxyphenyl-β-d-glucopyranoside-6P.
b
O-hydroxy-methylphenyl-β-d-glucopyranoside-6P.
c
6-O-β-d-glucopyranosyl-d-glucopyranose-6P.