Skip to main content
. 2023 Jul 12;14:1212785. doi: 10.3389/fphys.2023.1212785

FIGURE 1.

FIGURE 1

Circulating HDL cholesterol is reduced in mice with combined hepatocyte deficiency of Nrf1 and Nrf2. (A) Study design. (B) Cholesterol level in plasma and in ApoB-depleted plasma (n = 5-6 males pooled with 5-7 females). (C) Representative Western blot of FPLC fractionated plasma showing enrichment of ApoA1 but not ApoB in fractions 8 and 9, identified with a red rectangle in panel. For ApoA1 analysis, samples from two Nfe2l1 flox/flox ; Nfe2l2 flox/flox mice, one Nfe2l1 flox/flox mouse, and one Nfe2l2 flox/flox mouse infected with AAV-GFP underwent examination as well as three Nfe2l1 flox/flox ; Nfe2l2 flox/flox mice, two Nfe2l1 flox/flox mice, and one Nfe2l2 flox/flox mouse infected with AAV-CRE. For ApoB analysis, samples from three Nfe2l1 flox/flox ; Nfe2l2 flox/flox mice infected with AAV-GFP and three infected with AAV-CRE underwent examination as well as one Nfe2l2 flox/flox mouse infected with AAV-GFP and one infected with AAV-CRE. (D) Cholesterol profiles in FPLC fractionated plasma (n = 5-6 males pooled with 5-6 females). The p-value was determined by t-test, adjusted for multiple comparison (**, p < 0.01; ***, p < 0.001). Data in B is Box and Whisker plot, with points showing the value of each biological replicate. Data in D is mean ± standard deviation.