FIGURE 2.

Combined hepatocyte deficiency of Nrf1 and Nrf2 alters the proteome of circulating HDL. (A, B) Proteome analysis of FPLC fractions 8 and 9 identified in Figure 1 for males and females (n = 5-6), comparing hepatocyte deficiency of Nrf1 and Nrf2 (AAV-CRE) to control (AAV-GFP). Entails principal component analysis (A) and a heat map showing log₂ fold change differences for the indicated proteins (B). Blue indicates downregulation and red indicates upregulation. (C, D) Representative Western blot of indicated proteins in total plasma collected from male mice (C) and corresponding analysis (D) of these proteins (n = 4-6 males pooled with 4-6 females). The p-value was determined by t-test, adjusted for multiple comparison. Data in D is mean ± standard error of the mean, with points showing the value of each biological replicate.