FIGURE 3.

Altered liver gene expression correlates with altered circulating HDL proteome in mice with combined hepatocyte deficiency of Nrf1 and Nrf2. (A–C) Liver qPCR analysis for indicated gene expression, normalized by ribosomal protein 36b4 (n = 10-14 males pooled with 10–14 females), to assess liver expression of HDL-resident molecules involved in lipid metabolism (A), inflammation (B), and metal binding and clotting (C). The p-value was determined by t-test, adjusted for multiple comparison. Data in (A–C) are mean ± standard error of the mean with points showing the value of each biological replicate. (D) Correlation of log₂ fold change in HDL proteome with respect to log₂ fold change in liver gene expression, comparing combined hepatocyte deficiency of Nrf1 and Nrf2 to respective control. Correlational effect is indicated by r² value and p-value was determined using simple linear regression analysis. Each molecule is indicated, and its biological relationship color coded as shown in panel.