FIGURE 4.

Combined hepatocyte deficiency of Nrf1 and Nrf2 impairs HDL functionality. (A–F) Study designs (A, C, E) and results (B, D, F) of HDL capacity to accept and carry cholesterol that has undergone efflux from RAW264.7 macrophage (A, B), anti-inflammatory capacity to suppress TNFα-induced Vcam1 mRNA expression in C166 endothelial cells (C, D), and anti-inflammatory capacity to suppress LPS-induced Mcp1 mRNA expression in RAW264.7 macrophage (E, F), comparing mice with deficiency for Nrf1, Nrf2, or both (AAV-CRE) to respective control (AAV-GFP) mice (n = 5-6 males pooled with 5-7 females). The p-value was determined by t-test, adjusted for multiple comparison. Data in (B, D, F) are mean ± standard error of the mean, with points showing the value of each biological replicate. (A, C, E) were created with Biorender.com.