Equilibration buffer	Elution buffer A
100 mM K2HPO4	100 mM K2HPO4
2 mM EDTA	2 mM EDTA
200 mM sodium sulfate	70 mM sodium sulfate
Adjust pH to 7.2 with 1M KOH	Adjust pH to 7.2 with 1 M KOH
First-wash buffer	Elution buffer B
100 mM K2HPO4	100 mM K2HPO4
2 mM EDTA	2 mM EDTA
135 mM sodium sulfate	Adjust pH to 7.2 with 1 M KOH
Adjust pH to 7.2 with 1 M KOH