Figure 6: Effects of human Aβ40 or Aβ42 oligomers on the expression of neurotrophic factors and neuronal regeneration markers in SH-SY5Y cells.
Representative immunoblots (A and C) and densitometric quantitative analysis (B and D) showing protein levels of BDNF, NGF, and GAP-43 (B-D) in total protein lysates from human neuroblastoma cells (SH-SY5Y) stimulated with human Aβ40 (left) or Aβ42 (right) oligomers for 16 h. GAPDH levels were used as a loading control. n=9 biological replicates. Data are presented as a mean±SEM. *P<0.05, **P<0.01, ***P<0.001 and vs WT. Student t-tests have been performed between the 2 groups. (NT= Not Treated).