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[Preprint]. 2025 Jun 4:2023.07.11.548558. Originally published 2023 Jul 11. [Version 3] doi: 10.1101/2023.07.11.548558

Figure 3. Effects of human Aβ40 or Aβ42 oligomers on neurotrophic factors in neuronal cells (SH-SY5Y) and human cardiomyocytes (AC16).

Figure 3.

(A-D): Representative immunoblots (A-C) and densitometric analysis (B-D) of multiple independent experiments to evaluate BDNF, NGF, and GAP-43 protein levels (B-D) in human neuroblastoma cells (SH-SY5Y) stimulated with human Aβ40 or Aβ42 oligomers for 16 h. GAPDH levels were used as a loading control. (E-F): Representative immunoblots (E) and densitometric quantitative analysis (F) showing protein levels of BDNF and NGF in total protein lysates from human cardiomyocytes (AC16) stimulated with human Aβ40 oligomers for 16 h. GAPDH levels were used as a loading control. n=6–9. Data are presented as a mean±SEM. *P<0.05 and vs NT. Student t-tests have been performed between the groups. (NT= Not Treated).