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[Preprint]. 2023 Jul 10:2023.07.10.548325. [Version 1] doi: 10.1101/2023.07.10.548325

Figure 1: Assembly of the small subunit head is hierarchical.

Figure 1:

(A) Summary of the assembly hierarchy of ribosomal proteins to the head of the small ribosomal subunit. Assembly order was garnered from cryo-EM structures (indicated with the PDB ID at the bottom). Each intermediate is highlighted with a different color. Ribosomal proteins are indicated with the eukaryotic nomenclature. Contacts between different ribosomal proteins are indicated with line connections between the proteins, and arrows denote assembly progress. (B) A hierarchical set of steps regulates the formation of 80S-like ribosomes. Adapted from [21]. This cascade is initiated from stable pre-40S intermediate via phosphorylation of Ltv1 by the kinase Hrr25. While phosphorylation is necessary for Ltv1 release, it is not sufficient, and temporally separated. An unknown step that activates Ltv1 release was previously identified via its sensitivity to the Tsr1_RK, Rio2_K105E and Rio2_loop mutation [21].