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[Preprint]. 2023 Jul 10:2023.07.10.548325. [Version 1] doi: 10.1101/2023.07.10.548325

Figure 3: Ltv1 binding to pre-40S ribosomes.

Figure 3:

(A) A structure for Ltv1 was obtained from the alpha-fold website (https://alphafold.ebi.ac.uk/) and placed on the structure for pre-40S ribosomes (PDB ID 6FAI), by overlay with the Enp1-binding alpha-helix. The composite structure does not produce significant clashes with pre-40S ribosomes and shows interactions between Ltv1 and Rps3, Rps15 and Rps20. All structure displays were generated with Chimera [35]. (B) Detail of the complex demonstrating that Ltv1 separates Rps20_DE from Rps3_KK (highlighted in cyan and purple spheres, respectively), consistent with previous biochemical and genetic data. (C) Detail of the complex demonstrating that Ltv1 binds Rps3K75R76 (Rps3_KR, highlighted in purple spheres). (D) Doubling times (normalized to wt Rps3) for yeast cells expressing either wt Rps3 or Rps3_KR and either wt Ltv1 or Ltv1_L216S. Significance was tested using an unpaired t-test. ****, P<0.0001. (E) Detail of the complex demonstrating that Ltv1 binds Rps12, with a contact from Rps12_R108 (highlighted in red sphere). (F) Doubling times (normalized to wt Rps12) for yeast cells expressing either wt Rps12 or Rps12_R108A and either wt Ltv1 or Ltv1_L216S. Significance was tested using an unpaired t-test. ****, P<0.0001.