FIG. 5.
Expression of an alb-lacZ fusion constructed by creation of the alb::pMUPE1 insertion. Cultures were grown in DSM or DSM-G, and 1-ml samples were collected at either 30-min or 1-h intervals. β-Galactosidase activity was determined and plotted versus time. T0 indicates the end of the exponential growth phase. (A) Map of the alb operon and location of the lacZ fusion generated by recombination between the DNA of the alb locus and pMUPE1. Below the map is the expression profile of alb-lacZ in cells of strain ORB3147 grown in DSM and DSM-G. (B) Location of the sbo::neo-1 and sbo::neo-2 insertions with respect to the alb::pMUPE1 lacZ fusion. Also shown below the map is the expression profile of alb-lacZ in strains ORB3152 (sbo::neo-2) and ORB3153 (sbo::neo-1). (C) Expression profile of the albG-lacZ of strain ORB3231 (alb::pMUALBG) and its sbo::neo-1 (ORB3284) and sbo::neo-2 (ORB3230) mutant derivatives. (D) Effect of an abrB::neo insertion on the expression profile of alb-lacZ (alb::pMUPE1).