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Purification of subtilosin A
| Purification step | Vol (ml) | Total protein (μg) | AUa
|
Recovery (%) | Fold purification | |
|---|---|---|---|---|---|---|
| Total | Specific (AU/μg) | |||||
| Culture supernatant | 250 | 13,000 | 5,000 | 0.38 | 100 | 1 |
| Fractions from High Q column | 60 | NDb | 1,600 | ND | 32 | ND |
| Butanol extraction | 15 | 400 | 1,024 | 2.56 | 20.5 | 6.7 |
| Suspension after LH-20 chromatography | 0.4 | 240 | 640 | 2.7 | 12.8 | 7.1 |
AU, arbitrary units.
ND, not done.
