Figure 5. Strains vary in ENA copy number and expression.

A) Relative DNA-seq read count across a representative ENA gene (left trace, orange arrow) and three representative single-gene copies (right trace, grey arrows) in each of the three wild strains (see Methods). The plot shows the running average across 500bp windows from left to right along each sequence. As a control, sequence is shown for a related laboratory strain W303, sequenced with the same pipeline as the wild strains and known to carry four ENA copies. B) Average and standard deviation (n=3) of log₂ fold-change in ENA1 mRNA abundance before and after NaCl treatment, normalized to NCYC3290 basal levels at time 0 min.