Figure 3. Peptide library screens identify sequences with enhanced SHP2^T42A binding.

(A-C) Comparison of enrichment scores for N-SH2^WT and each N-SH2 mutant. (D) Comparison of enrichment scores for C-SH2^WT and C-SH2^E139D. For panels (A)-(D), the red line denotes where the wild-type and mutant SH2 have the same enrichment values. R² values are derived from linear regression analysis. For each SH2 domain, data are the average of two pTyr-Var Library screens and three Human pTyr Library screens. Source data for panels (A)-(D) can be found in Table S3. (E) Enrichment scores from peptide display screens for three representative peptides that showed enhanced binding to N-SH2^T42A relative to N-SH2^WT. (F) Binding affinity measurements for the three peptides shown in panel (E). N = 3 independent protein, peptide, and fluorescent peptide titrations.