Figure 2. K_D measurements reveal sequence-specific enhancement of binding affinity in N-SH2^T42A.

(A) Measured binding affinities of N-SH2^WT against peptides derived from various known SHP2 interactors. (B) Fold-change in K_D for N-SH2^T42A compared to N-SH2^WT, for each of the peptides shown in panel (A). (C) Same as (B), but for N-SH2^L43F. (D) Same as (B), but for N-SH2^T52S. For (A)-(D), N = 3–4 independent protein, peptide, and fluorescent peptide titrations. Source data and p-values can be found in Table S2.