Figure 3. Peptide library screens identify sequences with enhanced N-SH2^T42A binding.

(A-C) Comparison of enrichment scores for N-SH2^WT and each N-SH2 mutant. (D) Comparison of enrichment scores for C-SH2^WT and C-SH2^E139D. The red line denotes x = y. pTyr-Var Library screens (N = 2), Human pTyr Library screens (N = 3). Source data for panels (A)-(D) can be found in Table S3. (E) Enrichment scores from peptide display screens for three representative peptides that showed enhanced binding to N-SH2^T42A relative to N-SH2^WT. (F) Binding affinity measurements for the three peptides shown in panel (E) (N = 3).