Figure 1. Flow Diagram of Enrolled Patients.

ctDNA indicates circulating tumor DNA; NGS, next-generation sequencing; NSCLC, non–small cell lung cancer; and SCLC, small cell lung cancer.

^aThree patients with insufficient tissue for diagnosis had a plasma variant detected (STK11, KRAS^G12V, and PTEN).

^bThree patients with no biopsy had a plasma variant detected (TP53, U2AF1, and ERBB2 [formerly HER2 or HER2/neu] amplification).

^cThree patients with stage IA NSCLC (1 benign pleural effusion, 1 benign bone lesion, and 1 benign liver cyst).

^dNine patients had unresectable stage III disease (6 adenocarcinoma and 3 squamous carcinoma).

^eNo patients with squamous carcinoma or atypical cardinoid histologic characteristics had informative plasma results.

^fOf 7 patients with an actionable alteration in tissue only, 6 had undetectable ctDNA.

^gOf 11 patients with an actionable alteration in plasma only, 6 had insufficient tissue for NGS.