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[Preprint]. 2023 Jul 13:2023.07.13.548758. [Version 1] doi: 10.1101/2023.07.13.548758

Figure 7. EGFP mRNA transfects human fRPE in vitro.

Figure 7.

Human fRPE cells treated with EGFP mRNA encapsulated in 2TLNPs for 2 hours express EGFP. (A) fRPE cells treated with diluent only (same volume as (B)) have minimal EGFP expression. (B) Cells were treated with 0.1 mg/mL mRNA LNP and have higher GFP expression than those treated with diluent only. (C) fRPE cells treated with 0.5 mg/mL mRNA LNP also have EGFP expression higher than (A) and additionally have nuclear EGFP expression. Images shown are representative of at least 3 cell culture wells and are MIPs (scale bar: 50 μM). (D) EGFP expression was quantified and plotted in a violin plot as Corrected Total Cell Fluorescence (CTCF). P-values from an ANOVA test with post-hoc Tukey multiple comparisons are shown in the table. CTCF values are significantly higher in LNP-treated cells than in diluent-treated cells.