Figure 4. Linoleic acid diols activate calcium flux in cultured adipocytes.

(A) FLUOFORTE quantification in differentiated WT1 brown adipocytes treated after timepoint 2 with different concentrations of 9,10-diHOME or vehicle control. (B) FLUOFORTE quantification in differentiated WT1 brown adipocytes treated after timepoint 2 with different concentrations of 12,13-diHOME or vehicle control. (C) FLUOFORTE quantification in differentiated 3T3-L1 white adipocytes treated after timepoint 2 with different concentrations of 9,10-diHOME or vehicle control. (D) FLUOFORTE quantification in differentiated 3T3-L1 white adipocytes treated after timepoint 2 with different concentrations of 12,13-diHOME or vehicle control. Each timepoint is approximately 50 seconds apart. Data are plotted as the normalized means ± s.e.m.; $n=6$ wells per group; *P < 0.05 vs. vehicle by ANOVA with post-hoc Bonferroni test.