Figure 4.

3D diffusion dominates inner nucleosomal target search. a, D. melanogaster hsp70 nucleosome construct for investigating GAF-DBD target search when both cognate motifs are embedded in the nucleosome. b, Idealized single-molecule trajectories for GAF-DBD locating cognate motifs on the hsp70 nucleosome. c-e, Representative single-molecule trajectories on the hsp70 nucleosome, with corresponding FRET schematic shown below the trajectory. f, Binding event categories on the hsp70 promoter nucleosome construct. g, 1-CDF of motif dwell times on nucleosome (orange) compared with free DNA (blue). Free DNA data are from Cy7-Motif 1 dwell time at 6.25 mM MgCl₂ (Fig. 2e).