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[Preprint]. 2023 Jul 15:2023.07.14.549009. [Version 1] doi: 10.1101/2023.07.14.549009

PMC10369947.1; 2023 Jul 15
PMC10369947.2; 2024 May 25

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Extended Data Figure 1. — Purification of GAF-DBD protein. a, Subtractive Ni-NTA purification after “one-pot” reaction where 6xHis-SUMO was cleaved off and the N-terminal cysteine is exposed and labeled with Cy3. The calculated molecular weights are 34.4 kDa for 6xHis-SUMO-Cys-GAF-DBD, 21.0 kDa for Cys-GAF-DBD and 13.4 kDa for 6xHis-SUMO. b, Schematics of full-length GAF short isoform, GAF-DBD and NURF-binding regions⁶² to scale.