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[Preprint]. 2023 Jul 15:2023.07.14.549009. [Version 1] doi: 10.1101/2023.07.14.549009

PMC10369947.1; 2023 Jul 15
PMC10369947.2; 2024 May 25

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Extended Data Figure 2. — Donor-only dwell time is inversely correlated with ionic strength. a, Representative single-molecule trajectories when Cy5-cognate DNA is bound by Cy3-GAF-DBD in 0, 25, 50 or 100 mM NaCl. Grey highlighted durations indicate donor-only dwell times. b, Representative trajectories when Cy5-cognate DNA was bound by Cy3-GAF-DBD in 0, 1, 5, 7.5, or 10 mM MgCl₂. c, Donor-only dwell time (τ from fitting 1-CDF to a single-exponential decay; see Material and Methods) depends on NaCl concentration. d, Donor-only dwell time also depends on MgCl₂ concentration. Error bars are standard error from fitting.