Extended Data Figure 4.

GAF-DBD preferentially visits the same cognate motif on the same hsp70 nucleosome. a, Representative single-molecule fluorescence intensity time trajectories showing repetitive visits to the same binding site on a single nucleosome. Upper trace shows repetitive visits to binding site A; middle trace shows repetitive visits to binding site B; lower trace shows repetitive visits to binding site C. Black asterisks mark transient Cy3-only fluorescence within a binding event, potentially caused by short-range 1D diffusion on the nucleosome. Green asterisks indicate binding events to non-cognate sites on the nucleosome. b, For all binding events on site A (left pie chart, N = 65), B (middle, N = 45) or C (right, N = 25), the binding location of the following binding event.