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[Preprint]. 2024 May 25:2023.07.14.549009. Originally published 2023 Jul 15. [Version 2] doi: 10.1101/2023.07.14.549009

PMC10369947.1; 2023 Jul 15
PMC10369947.2; 2024 May 25

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Extended Data Figure 2. — Purification of GAF-DBD protein. a, Subtractive Ni-NTA purification after “one-pot” reaction which cleaved off 6xHis-SUMO and labeled the N-terminus of GAF-DBD with Cy3. The calculated molecular weights are 34.4 kDa for 6xHis-SUMO-Cys-GAF-DBD, 21.0 kDa for Cys-GAF-DBD and 13.4 kDa for 6xHis-SUMO. b, Schematics of GAF-FL short isoform, GAF-DBD and NURF-binding regions (PMID 11583616) to scale. c, Native hsp70 promoter DNA sequence. GAF cognate sites are highlighted in red. TATA box in bold.