Extended Data Figure 3.
Donor-only dwell time is inversely correlated with ionic strength of buffer solution. a, Representative trajectories showing Cy5-cognate DNA bound by Cy3-GAF-DBD in 0, 25, 50 or 100 mM NaCl. Grey-highlighted durations indicate donor-only dwell times. b, Representative trajectories showing Cy5-cognate DNA bound by Cy3-GAF-DBD in 0, 1, 5, 7.5, or 10 mM MgCl2. c, Donor-only dwell time (t from fitting 1-CDF to a single-exponential decay; see Methods) as a function of NaCl concentration, and d, MgCl2 concentration. Error bars are standard error from fitting. DNA is the same as cognate DNA in Fig. 1.