Extended Data Figure 4.

GAF sliding kinetics on Cy5 & Cy7 dual-labeled DNA as a function of salt concentration, cognate site and motif orientation. a, Representative single-molecule trajectories of Cy5 & Cy7 DNA bound by Cy3-GAF-DBD in low MgCl₂ (3 mM). b, Rastergram of 31 Cy5 & Cy7 DNA molecules bound by Cy3-GAF-DBD at 3 mM MgCl₂. c, Schematic of Site 2 Only construct where Site 1 was replaced with non-cognate sequence. d, Representative single-molecule trajectories of Site 2 Only DNA bound by GAF-DBD. e, Rastergram of 40 Site 2 Only DNA molecules bound by GAF-DBD. f, Schematic of the flipped motif DNA construct where Site 1 is replaced with its complementary sequence Site 1’. g, Representative single-molecule trajectories of flipped motif DNA bound by GAF-DBD. h, Rastergram of 57 flipped motif DNA molecules bound by GAF-DBD. DNA same as Fig. 4. i, Categories of GAF-DBD arrival landing site and departure launching site.