Table 1.
Chemical Shift Perturbation Statistics for Prostaglandins*
| Protein | Ligand | Mean CSP ± Standard Deviation for All Peaks (ppm) | Mean CSP ± Standard Deviation for Top 5% of Most Perturbed Peaks (ppm) |
|---|---|---|---|
|
| |||
| SRC1 PAS-B | PGB1 | 0.052 ± 0.055 | 0.229 ± 0.023 |
| PGE2 | 0.031 ± 0.031 | 0.129 ± 0.016 | |
| PGA1 | 0.036 ± 0.046 | 0.198 ± 0.029 | |
| PGE1 | 0.032 ± 0.033 | 0.134 ± 0.021 | |
|
| |||
| SRC1 PAS-BNurr1 AF128–51 fusion | PGB1 | 0.026 ± 0.026 | 0.114 ± 0.036 |
| PGE2 | 0.009 ± 0.007 | 0.031 ± 0.007 | |
| PGA1 | 0.014 ± 0.019 | 0.083 ± 0.027 | |
| 8-iso PGE1 | 0.013 ± 0.010 | 0.041 ± 0.019 | |
| PGD1 | 0.008 ± 0.004 | 0.019 ± 0.002 | |
| 15d-PGJ2 | 0.038 ± 0.064** | 0.270 ± 0.076** | |
*:
values are for 1:10 protein:ligand ratio and 0.2 mM protein concentration
**:
values are for 1:2 protein:ligand ratio after overnight incubation at 4 °C with 0.2 mM protein