Figure 3. Immune response and bacterial burden in response to immunizing BALB/c mice with various doses of S. typhimurium SL3261 expressing P. aeruginosa serogroup O9.
Anti-P. aeruginosa serogroup O9 serum (A) IgM and (B) IgG antibody response after intranasal vaccination of BALB/c mice to various doses of vector or vaccine. (C) Bacterial load in the nasal wash and lungs of intranasally immunized mice 24 hours post-challenge with 4.5 × 107 CFU of PAO9. All samples were plated for viable CFU on PIA. Each point represents a single mouse. Data were analyzed by one-way ANOVA, * P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. Error bars represent the mean and SEM.

