Figure 4. Refining epitopes of the nanobody repertoire.

All epitopes are mapped on the structure of the RBD (PDB ID: 6M0J). (A) The epitopes of six RBD nanobody groups (colored dark gray) able to bind/neutralize one or more variants. Four of these epitope groups: I, III, IV and V were further refined resulting in an additional six epitope groups - creating a total of 12 epitope groups able to bind one or more variants of concern. bind/neutralize the circulating variants XBB and BQ.1 and BQ.1.1. All structure representations were created (B) Summary of variant specific and pan-variant nanobodies. (C) Nanobody groups predicted to on ChimeraX (Pettersen, Goddard et al. 2021).