Figure 7. Nanobodies effective against circulation variants of concern.
The nanobody epitopes (red) that retain effectiveness against wild-type (PDB ID: 7KNB), delta (PDB ID: 7V7O), omicron BA.1, omicron BA.4 (the nanobody epitopes were mapped on to PBD ID: 7XO5 for both BA.1 and BA.4 due to the lack of a suitable BA.4 structure),omicron XBB.1 and BQ.1.1 (the nanobody epitopes were mapped on to PBD ID: 8IOU for both XBB.1 and BQ.1.1 due to the lack of a suitable BQ.1.1 structure). The spike trimer (silver) where glycans are represented as light blue sticks for each variant is displayed in three views: top = side-view of spike trimer; middle = birds-eye view looking down on the S1 domain and; bottom = birds-eye view (same as middle view), with the S1 domain rendered transparent to enable visualization of the S2-domain. All structure representations were created on ChimeraX (Pettersen, Goddard et al. 2021).
