Figure 2. Treatment of SMC (Myh11-CreER^T2-eYFP) and EC (Cdh5-CreER^T2-eYFP)-lineage tracing Apoe^−/− mice with advanced atherosclerotic lesions with ABT-263 had no effect on lesion size but increased mortality.

(A) Experimental design, SMC- and EC-lineage tracing Apoe^−/− mice were fed a WD for 18 weeks followed by ABT-263 treatment on western diet (WD) for 6 weeks. (B) Probability of survival (Kaplan-Meier curve). (C) Representative 10x images with 100μm scale bar of MOVAT staining on Brachiocephalic Artery (BCA). (D) Lesion area from D. (E) Lumen area from D. (F) External elastic lamina (EEL) area from D, for outward remodeling. (G) Aortic roots stained with MOVAT. (H) Lesion area quantification from G. (I) Representative 10x images with 100μm scale bar of Picrosirius red staining on Brachiocephalic Artery (BCA). (J) Quantification of matured (red) collagen content normalized to lesion area from figure I. A repeated measures Two-way ANOVA method was used for statistical analysis in D-F whereas Mann-Whitney U-tests were used for H and J. Biologically independent animals are indicated as individual dots, error bars show the SEM, Mantel-Cox test used for B. The p-values are indicated on the respective graphs.