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[Preprint]. 2024 Sep 29:2023.07.11.548587. Originally published 2023 Jul 11. [Version 2] doi: 10.1101/2023.07.11.548587

Figure 3: Design and extraction of L-Series Substrates.

Figure 3:

A) Design of L-series substrates. The length of the TMD is calculated by assuming a rise of 1.5 Å per residue. GRAVY score was calculated by inserting the TMD sequence into the GRAVY calculator (https://www.gravy-calculator.de/). The ΔG for TMD insertion was calculated using the website (https://dgpred.cbr.su.se/index.php?p=TMpred)25,26. TMD sequences for L-series substrates all start and end with Leucine.

B) L-series substrates with a predicted hydrophobic mismatch show enhanced extraction activity. L-series substrates were reconstituted in standard liposomes.

C) Protease protection assay of L-series substrates in standard liposomes.