Figure 5: Msp1 extraction activity is enhanced with increasing membrane fluidity.
A) Composition of fluidity series liposomes.
B) Lipid packing of F-series liposomes measured by C-Laurdan spectroscopic measurements. Generalized polarization (GP) values range from +1 (most ordered) to −1 (most disordered).
C) Extraction of standard Sumo-Sec22 substrate in F-series liposomes.
D) Protease protection assay for proteoliposomes in C.