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[Preprint]. 2024 Sep 29:2023.07.11.548587. Originally published 2023 Jul 11. [Version 2] doi: 10.1101/2023.07.11.548587

Figure 2: Msp1 extraction activity correlates with hydrophobic mismatch between the substrate TMD and lipid bilayer.

Figure 2:

A) Composition and thickness of T-series liposomes.

B) PEG-maleimide labeling shows increased TMD exposure in thinner liposomes.

C) Quantification of data from B from 3 separate reconstitutions. Error bars show standard error of the mean. The amount of labeled material in the + detergent sample is defined as 100% labeling.

D) Extraction of the standard Sumo-Sec22 substrate in T-series liposomes. Msp1 shows more robust extraction activity in thinner liposomes.

E) Protease protection assay of standard Sumo-Sec22 substrate in T-series liposomes.