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[Preprint]. 2023 Aug 2:2023.07.11.548598. Originally published 2023 Jul 11. [Version 2] doi: 10.1101/2023.07.11.548598

Figure 3. Structural basis of DDM1-nucleosome interactions.

Figure 3.

(A) Protein domain schematic of DDM1. Residue numbers indicate the boundaries of DDM1 and its domains: the N-terminal DEXD ATPase domain and helicase superfamily C-terminal domain (HELICc). Dashed lines represent the coverage of the DDM1 molecular model. (B) Overview of the molecular structure of the DDM1-nucleosome complex as determined by cryo-EM. DDM1 domains, corresponding to the two lobes, are labeled on the side view. (C) DDM1-histone interactions. The experimental density map of the complex shows that DDM1 interacts with histones H3.3 (green) and H4 (yellow). For the inset, a cartoon representation with partially transparent cryoEM map colored by domains is shown. Amino acids along the DDM1-histone interface (6 Å cutoff) are displayed as sticks, and include T80 and D81 of histone H3.