Figure 3. ShyAL109K overexpression restores normal cell growth and morphology in ΔdacA1 cells grown on LB media.

(A) Overnight cultures were grown on salt-free LB and then spot-tittered on either LB or salt-free LB with 1mM IPTG. (B) Overnight cultures were sub-cultured 1:100 in either LB or salt-free LB at 37°C until they reached exponential phase, followed by addition of inducer (1mM IPTG). Phase contrast images were taken after 2 hours of induction. (C) Cells were segmented using Omnipose, and area, width and length were calculated with MicrobeJ. Statistical significance was determined with Welch’s two sample t-test. ****, P < 0.0001. Shown are boxplots with raw data points.